| 高雪岩王文全△ 孙建宁刘思秦郑巧云.赤芍总苷抗氧化活性及对大鼠肝星状细胞增殖的影响[J].,2011,11(14):2609-2611 |
| 赤芍总苷抗氧化活性及对大鼠肝星状细胞增殖的影响 |
| Study on the Antioxygen Activity of Total Paeony Glucosides and ItsInhibition Effect on the Proliferation of HSC-T6 |
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| DOI： |
| 中文关键词: 赤芍总苷 DPPH 法 抗氧化 肝星状细胞 增殖 |
| 英文关键词: Total paeony glucosides DPPH method Antioxygen HSC-T6 Proliferation |
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| 中文摘要: |
| 目的：研究赤芍总苷体外抗氧化活性及对大鼠肝星状细胞HSC-T6 细胞增殖的影响。方法：采用DPPH 法测定赤芍总苷体外
清除DPPH 自由基及抗氧化活性，根据清除率曲线，确定EC50 值；采用MTT 法考察赤芍总苷对大鼠肝星状细胞HSC-T6 细胞增
殖的影响，确定起效剂量，并考察其量效关系。结果：赤芍总苷对DPPH 自由基具有较强清除作用，其对DPPH 的清除率的回归方
程为y=7.4432x+0.6111 （r=0.9967），EC50 值为6.64 mg/L；赤芍总苷对HSC-T6 的增殖具有明显的抑制作用，给药浓度为1.152
mg/mL 时，其对HSC-T6 细胞增殖的抑制率可达39.240 %。结论：赤芍总苷具有较强的抗氧化活性，对肝星状细胞的增殖有一定抑
制作用。 |
| 英文摘要: |
| Objective: To ingvestigate the antioxygen activity of total paeony glucosides and its inhibition effect on the proliferation
of HSC-T6. Methods: The in vitro antioxygen activity of total paeony glucosides was determined by DPPH method, and EC50 was
caltulated according to the clearance rate curve. And MTT method was employed to investigate the inhibition effect on proliferation of
HSC-T6, its effective dose was determined and dose-effect relationship was inspected. Results: Total paeony glucosides had great scavenging
effect towards DPPH free radical. The regression equation was y=7.4432x+0.6111 (r=0.9967), and its EC50 was 6.64 mg/L. There
was inhibition effect on the proliferation of HSC-T6. The inhibiton ration reached was 39.240 %, when the concentration was 1.152
mg/mL. Conclusions: Total paeony glucosides had strong antioxidant activity and great inhibition effect on the proliferation of hepatic
stellate cells. |
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