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目的：探讨补肾法与舒肝法对大鼠吗啡精神依赖的调节作用及机制。方法：使用盐酸吗啡建立大鼠精神依赖模型（conditioned place preference, CPP），采用Obersiver 5.0 行为学软件分析大鼠的CPP 效应，并利用Real time PCR 方法测定伏核和前额叶皮质内NR2B 亚基的mRNA 含量。结果：与对照组相比，模型组大鼠在白侧累积停留时间极显著长于对照组（P＜0.01），同时伏核与前额叶皮质中NR2B 的mRNA 水平也显著升高（P＜0.01 和P＜0.05）；与模型组相比，补肾组和舒肝组大鼠在白侧的累积停留时间显著下调（P＜0.05），疏肝组NR2B 的mRNA 也显著下降（P＜0.05），而补肾组变化不显著。结论：①补肾法与舒肝法对吗啡引起的精神依赖均有调节作用。②舒肝法的作用机制可能在于通过影响伏核和前额叶皮质中NR2B 亚基的mRNA 表达进一步调节精神依赖的相关神经通路。③补肾法的作用机制与NR2B 亚基的mRNA 表达关系不密切，其调节的具体机制尚有待研究。

英文摘要:

Objective: To analyze the effect and mechanism of liver-smoothing therapy and kidney-nourishing therapy to morphine-induced conditioned place preference. Methods: Morphine was injected to set up the conditioned place preference model in rats, Obersiver 5.0 Behavioral Software was used to analyze the time of staying in morphine-paired compartment，and real time PCR was used to detect the mRNA of NR2B. Result: The morphine model group, compared to the control group, the time of staying in morphine-paired compartment were significantly up regulated (P＜0.01), and the mRNA of NR2B in nucleus accumbens and prefrontal cortex were up regulated as well (P＜0.01 and P＜0.05). Compared to the morphine group, in the liver-smoothing therapy group, both the time of staying in morphine-paired compartment and mRNA were significantly reduced (P＜0.05), but in the kidney-nourishing therapy, only the time of staying in morphine-paired compartment were significantly reduced (P＜0.01). Conclusion: ①The morphine-induced conditioned place preference can be regulated by both liver-smoothing therapy and kidney-nourishing therapy. ② The regulating mechanism of liver-smoothing therapy to morphine-induced conditioned place preference may be related to the level of mRNA of NR2B in nucleus accumbens and prefrontal cortex. ③The regulating mechanism of kidney-nourishing therapy to morphine-induced conditioned place preference should not be related to the level of mRNA of NR2B in nucleus accumbens and prefrontal cortex.

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