| 齐凤琴李廷利△ 张博张冰郭冷秋黄莉莉.RP-HPLC 法测定酸枣仁汤中芒果苷的含量[J].,2011,11(12):2353-2355 |
| RP-HPLC 法测定酸枣仁汤中芒果苷的含量 |
| Determination of Mangiferin in Suanzaoren Decoction by RP-HPLC |
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| DOI： |
| 中文关键词: 酸枣仁汤 芒果苷 RP-HPLC |
| 英文关键词: Suanzaoren Decoction Mangiferin RP-HPLC |
| 基金项目:教育部2008 年博士点基金（200802280001） |
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| 中文摘要: |
| 目的：建立测定酸枣仁汤中芒果苷含量的反高效液相色谱分析方法。方法：色谱柱：WatersC18 (250nm×4.6mm,5μm)；流动
相：乙腈-0.1%磷酸水溶液，梯度洗脱；流速：1.0ml/min，检测波长：257nm。结果：芒果苷在0.316μg-1.264μg 范围内与峰面积线性
关系良好（r=0.9999），平均加样回收率为97.64%，RSD 为0.36%。结论：本方法简便、准确、可靠、重复性好，可作为酸枣仁汤中芒
果苷的含量测定方法。 |
| 英文摘要: |
| Objective: To establish a HPLC method for the determination of mangiferin in Suanzaoren Decoction. Methods: The
determination was performed on WatersC18 column (250nm×4.6mm,5μm)with a grient mobile phase composed of acetionitrile and
0.1% phosphoric acid. The detection wavelength was 257nm,and the flow rate was 1ml/min. Results: The Mangiferin showed good linearity（
r=0.9999）in the range of 0.316μg-1.264μg, the average recovery was 97.64% with RSD 0.36%. Conclusion: The method is simple,
accurate, reliable and suitable for determination of Mangiferin in Suanzaoren Decoction. |
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