| 张少峰谢胜甘伟罗茂华李云飞.糖尿病性膀胱病逼尿肌中SCFmRNA 和SCF 蛋白的表达[J].,2011,11(12):2317-2320 |
| 糖尿病性膀胱病逼尿肌中SCFmRNA 和SCF 蛋白的表达 |
| Expression and Significance of SCF Messenger Ribonucleic Acid and SCFProtein in the Diabetic Cystopathy |
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| DOI： |
| 中文关键词: Cajal 样细胞 糖尿病性膀胱病 SCF SCF/c-kit 信号通路 |
| 英文关键词: Cajal-like cells Diabetic cystopathy SCF/c-kit signal pathway |
| 基金项目: |
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| 摘要点击次数: 826 |
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| 中文摘要: |
| 目的：检测DCP 逼尿肌中SCF 表达水平，探讨SCF 基因表达与DCP 关系及其发病机制。方法：按1：2 病例对照研究，采用
链脲佐菌素(STZ) 及尿动力学检测成功建立DCP 豚鼠20 只为实验组，并以同质豚鼠40 为对照组，应用RT-PCR 和
Western-blotting 方法分别检测各组膀胱逼尿肌中SCF mRNA、SCF 蛋白的表达。结果：DCP 豚鼠组织中SCF mRNA 表达与正常
对照组比较无明显显著差异（P＞0.05），DCP 豚鼠组织中SCF 蛋白表达明显低于正常对照组（P＜0.01）。结论：DCP 组织中SCF
蛋白表达减少与SCF 基因翻译水平异常有关，因此高血糖环境下SCF 基因表达异常可能是DCP 的发病机制之一。 |
| 英文摘要: |
| Objective: The aim of this study was to determine the expression of SCF mRNA and SCF protein in the bladders in
guinea pigs of diabetic cystopathy (DCP)．To explore the correlation and mechanisms between SCF gene expression and DCP．
Methods: Sixty guinea pigs were divided randomly into the normal group ( n=40) and the experimental group ( n=20) , the guinea pigs of
the experimental group were injected with streptozotocin (STZ) to induce DCP model．Expression of SCF mRNA was detected by
reverse transcription polymerase chain reaction (RT-PCR) and expression of SCF protein was tested and analyzed by Western-blotting．
Results: It is no significant difference (P> 0.05) that DCP group of SCFmRNA expression compared with control group, SCF protein
expression significantly declined in the DCP group．Conclusion: The declined expression of SCF gene at translation phases destroys the
SCF/c-kit signal pathway, which lead to the dysfunction of Cajal-like cells in DCP of guinea pig，so the abnormal expression of SCF gene
is one of pathogenesis of DCP |
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