| 吴勇军1,2 唐仪1 冯德云3 李筝1 张漾1 赵毅1 伍镇江1 苏琦2.CD151 对甲状腺癌细胞迁移及侵袭能力的影响[J].,2011,11(11):2071-2074 |
| CD151 对甲状腺癌细胞迁移及侵袭能力的影响 |
| Impact of CD151 on Migration and Invasion of Human Thyroid CancerCell Line CGTHW-1 |
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| DOI： |
| 中文关键词: 甲状腺癌细胞 CD151 迁移 侵袭 |
| 英文关键词: Thyroid cancer cell CD151 Migration Invasion |
| 基金项目:湘潭市科技计划项目（JZ200704） |
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| 中文摘要: |
| 目的：构建CGTHW-1/ pGenesil-1-CD151shRNA 稳转细胞系和空载体细胞系CGTHW-1/ pGenesil-1，探讨CD151 对人甲状
腺癌CGTHW-1 细胞迁移及侵袭的影响及其作用机制。方法：应用Lipofectamine2000 将真核干扰载体pGenesil-1 -CD 151 shRNA
和空载体pGenesil-1 导入甲状腺滤泡癌细胞CGTHW-1，经卡那霉素抗性筛选得到稳定的克隆并扩大培养成细胞系，Western blot
检测CD151 在CGTHW-1 细胞中的表达；划痕实验和Transwell 实验分别观察CD151 对细胞迁移和侵袭能力的影响。结果：成功
建立了CGTHW-1/ pGenesil-1-CD151shRNA 稳转细胞系和空载体细胞系CGTHW-1/pGenesil-1，Western blot 结果显示CD151 在
CGTHW-1 细胞中表达，干扰CD151 基因后，CGTHW-1 细胞的迁移和侵袭能力明显降低。结论：将CD151 基因干扰后可明显抑
制甲状腺癌细胞的迁移和侵袭能力。CD151 可能是影响甲状腺癌侵袭转移的重要因子之一。 |
| 英文摘要: |
| Objective: To construct the vector of CGTHW-1/ pGenesil-1- CD151shRNA for interference and to investigate the impact
of CD151 on the migration and invasion of CGTHW-1 cell in thyroid cancer. Methods: The eukaryotic interference plasmid pGenesil-
1-CD151shRNA, and empty vector pGenesil-1 were transfected into CGTHW-1 cell. The cells were selected by kanamycin. After
infection, CD151 expression in CGTHW-1 cells was observed by Western blot. Cell migration and invasion were detected by Wound
Healing assay and Transwell assay. Results: CGTHW-1/pGenesil-1- CD151shRNA and CGTHW-1/pGenesil-1cells were succ- essfully
set up. Western blot results showed that CD151 expression in CGTHW-1 cells. Wound Healing assay and Transwell assay showed that
CD151 gene interfered , CGTHW-1 cells significantly reduced migration and invasion. Conclusion: The interference of CD151 gene can
significantly inhibit the migration and invasion of thyroid cancer cells. CD151 is likely one of the important factors to affect invasion and
metastasis of thyroid cancer. |
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