| 陈健1 徐艳1 王艳2 王强1 环飞1 肖杭1.Martentoxin 对TNF-α 引起脐静脉内皮细胞释放一氧化氮的影响[J].,2011,11(11):2045-2048 |
| Martentoxin 对TNF-α 引起脐静脉内皮细胞释放一氧化氮的影响 |
| Effects of Buthus Martensii Karsch Toxin Martentoxin in Human UmbilicalVein Endothelial Cells Releasing Nitric Oxide Induced by TNF-α |
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| DOI： |
| 中文关键词: 东亚钳蝎 Martentoxin 一氧化氮 脐静脉内皮细胞 |
| 英文关键词: BMK Martentoxin Nitric oxide HUVECs |
| 基金项目: |
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| 中文摘要: |
| 目的：研究东亚钳蝎毒素Martentoxin 对脐静脉内皮细胞释放NO 的影响。方法：人脐带经胶原酶消化分离后获得脐静脉内
皮细胞，经抗血管性血友病8 因子鉴定后, 用TNF-α 建立NO 释放模型，观察Martentoxin 对脐静脉内皮细胞释放NO 的影响。结
果：①经6，12，24h 作用后，10μM、100 μM Martentoxin 能降低TNF-α 引起的NO 释放增加(P<0.05)。②Martentoxin 降低TNF-α
引起的iNOS 活性增强并对TNF-α 引起的内皮源性一氧化氮合酶mRNA 下调具有一定保护作用(P<0.05)。结论：Martentoxin 抑
制TNF-α 引起的脐静脉内皮细胞释放NO 增加。 |
| 英文摘要: |
| Objective: To investigate the impact of Martentoxin on Human umbilical vein endothelial cells (HUVECs) releasing
nitric oxide induced by TNF-α. Methods: HUVECs were isolated and identified by Immunocytochemistry with Anti-von willebrand
Factor (vWF). The NO releasing model of HUVECs was established by treating with 10 ng/ml TNF-α and the effect of Martentoxin on
NO releasing was observed. Results: ①Application of 10 μM and 100μM Martentoxin i nhibits TNF-α-induced NO release after
exposure for 6 h, 12 h and 24 h (P<0.05). ② Martentoxin inhibits the activity of iNOS and retarded the down-regulation of eNOS mRNA
regulated by TNF-α (P<0.05). Conclusion: Martentoxin inhibits the NO production in HUVECs induced by TNF-α. |
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