| 郗永义胥照平高丽华邵勇胡显文△ 陈惠鹏.炭疽受体CMG2-Fc 融合蛋白在CHO 细胞中的表达、纯化与鉴定[J].,2011,11(11):2022-2025 |
| 炭疽受体CMG2-Fc 融合蛋白在CHO 细胞中的表达、纯化与鉴定 |
| Expression,Purification and Identification of Anthrax Receptor CMG2-FcFusion Protein in CHO Cell |
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| DOI： |
| 中文关键词: 炭疽杆菌 CMG2 炭疽毒素 融合蛋白 |
| 英文关键词: Bacillus anthracis CMG2 Anthrax toxin Fusion protein |
| 基金项目: |
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| 中文摘要: |
| 目的：构建炭疽受体CMG2 和人IgG1 Fc 片段融合基因载体，转染CHO 细胞并通过毒素中和试验检测CMG2-Fc 拮抗炭疽
毒素（PA+LF）的能力。方法：将含有CMG2 胞外区1-217AA 片度基因和人IgG1 的Fc 片段基因共同连接入pcDNA3.1 载体转染
CHO 细胞并筛选高表达CMG2-Fc 的CHO 细胞系，通过小鼠RAW264.7 巨噬细胞保护试验检测CMG2-Fc 拮抗炭疽毒素的能
力。结果：获得了表达CMG2-Fc 的细胞株，毒素中和实验显示该蛋白可以有效抑制炭疽毒素引起的细胞损伤。结论：CMG2-Fc 能
够保护小鼠巨噬细胞免受炭疽毒素攻击，提示其可以作为抗毒素治疗炭疽感染。 |
| 英文摘要: |
| Objective: To construct fusion gene vector of anthrax receptor CMG2 and human IgG1 Fc fragent, transfect CHO cell
and to testify the inhibit ability of CMG2-Fc on anthrax toxin (PA and LF) by toxin neutralization assay. Methods: An expression vector
including CMG2 (1-225AA) gene and Fc fragment of human immunoglobulin G (IgG) were constructed, and CHO cell line with higher
CMG2-Fc expression were got. The effect of CMG2-Fc on inhibiting anthrax toxin was detected by mouse macrophage protection assay.
Results: The CHO cell line expressing CMG2-Fc protein were got, and toxin neutralization assay showed that CMG2-Fc could protect
mouse RAW264.7 macrophage cell against anthrax toxins. Conclusion: CMG2-Fc can protect mouse macrophage against anthrax toxin
challenge, which indicated that it maybe used as drugs against anthrax in future. |
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