王国槐陈光尧张振乾官春云陈社员.甘蓝型油菜芥酸含量的SSR 标记分析[J].,2011,11(9):1683-1687 |
甘蓝型油菜芥酸含量的SSR 标记分析 |
Analysis of SSR Markers of Erucic Acid Content in Brassica Napus |
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DOI: |
中文关键词: 甘蓝型油菜 芥酸 SSR 标记 CB10364-h |
英文关键词: Brassica napus Erucic acid SSR marker CB10364-h |
基金项目:国家重点基础研究发展计划(973 计划)项目(2006CB101600)资助 |
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中文摘要: |
目的:探讨高芥酸材料与低芥酸材料杂交效果,为促进高芥酸油菜育种的研究。方法:采用高芥酸材料与低芥酸材料杂交的
F2 群体作为材料,研究其遗传性状,并对亲本间的芥酸含量进行了SSR 标记分析。结果:发现F2 群体中的单株芥酸含量受两对
基因控制,其遗传规律符合由一对基因控制的分离比例,得到CB10364、Ra2-E12 两个共显性标记。结论:CB10364 标记与芥酸含
量紧密连锁,单株带型为CB10364-a 的芥酸含量<6%,单株带型为CB10364-h 的芥酸含量6%~36%,带型为CB10364-b 的芥酸
含量>36%,能较好的区分群体的芥酸含量,该结果可促进高芥酸油菜的育种研究。 |
英文摘要: |
Objective: This research was studied to promote the development high erucic acid rape breeding. Method: F2 groups
which were hybridized by High erucic acid material and low erucic acid hybrid material were studied here, and the parents' erucic acid
content was analyzed by SSR markers. Results: The erucic acid content was controlled by two genes, and the inheritance rule accorded
with the segregation ratio which is controlled by one pair gene. Meanwhile, two codominant markers of CB10364 and Ra2-E12 were obtained.
Conclusion: CB10364 markers tightly linked with the erucic acid content. Individual plant strip type of CB10364-a has the erucic
acid content <6%, while the type of CB10364-h has the erucic acid content from 6% to 36%, and that of CB10364-b has the erucic acid
content > 36%. The results can be used on high erucic acid rapeseed breeding, and may be highly availability. |
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