| 徐丽莎1 刘文晶2 申少波3 杨照1 陈蕊蕊1 韩英1 尹芳1 时永全1 周新民1.蚕丝蛋白和明胶复合组织工程材料与肝细胞相容性的实验研究[J].,2011,11(8):1432-1435 |
| 蚕丝蛋白和明胶复合组织工程材料与肝细胞相容性的实验研究 |
| Experimental Study on Silk Protein - Three-dimensional Gelatin Scaffoldand Compatibility of Liver Cells |
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| DOI： |
| 中文关键词: MTT 比色法 蚕丝蛋白 明胶 肝组织工程 细胞相容性。 |
| 英文关键词: MTT Silk protein Gelatin Liver tissue engineering |
| 基金项目:国家863 计划(2009AA043801) |
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| 中文摘要: |
| 目的：研究蚕丝蛋白- 明胶三维材料支架对人永生化肝细胞系QZG 贴附及增殖的影响。方法: 采用四氮唑盐比色法(MTT)、
细胞计数法检测QZG 细胞在纯蚕丝生物材料上与在蚕丝蛋白- 明胶复合材料上的增殖情况，用扫描电镜观察QZG 细胞在两种
三维生物材料上的贴附与增殖情况。结果: QZG 细胞可以在蚕丝蛋白生物材料贴附及增殖，在引入明胶的蚕丝蛋白材料上细胞贴
附更紧密，增殖更明显。结论: 蚕丝蛋白与明胶复合材料支架具有良好的细胞贴附性能，通过改进在肝组织工程应用方面将具有
一定应用前景。 |
| 英文摘要: |
| Objective: To study the silk fibroin material's effect on proliferation of human normal hepatic cell line QZG，and to
testify the cytocompatibility when blending of silk fibroin and gelatin into 3D scaffold. Methods:The activity of cell proliferation and
relative growth rate (RGR) were assessed by MTT colorimetric assay and viable cell counting, The effect of silk protein materials on
growth of cells was observed by scanning electron microscopy (SEM). Results: Human normal hepatic cell line QZG were cultivated in
vitro directly on pure silk protein materials and silk fibroin/ gelatin materials. Cells solidly adhered to the materials. The activity of cell
proliferation on silk fibroin/ gelatin materials was significantly more than that in the group of pure silk fibroin materials. The result of
viable cell counting was compatible to MTT colorimetric assay. Conclusion:The silk fibroin materials together with gelatin has a good
cytocompatibility with human normal hepatic cell line QZG. It will be useful in liver tissue engineering. |
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