| 宋美华1 唐金海2△ 吴建中3 张晓梅3 曹海霞3.乳腺癌组织CCNA1 启动子区甲基化与肿瘤转移的相关性[J].,2011,11(7):1321-1326 |
| 乳腺癌组织CCNA1 启动子区甲基化与肿瘤转移的相关性 |
| Correlation of Cyclin A1 Gene Hypermethylation in Promoter Region andMetastasis of Breast Carcinoma |
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| DOI： |
| 中文关键词: 乳腺癌 Cyclin A1 基因 甲基化 预后 |
| 英文关键词: Breast cancer Cyclin A1 Methylation Prognosis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨CCNA1 基因甲基化在乳腺癌发生发展中的作用。方法：采用Qiagen-FFPE 的步骤提取95 例石蜡包埋乳腺癌组
织的基因组DNA，应用限制性酶切-PCR 对所提DNA 进行CCNA1 甲基化检测。结果：乳腺癌组织中CCNA1 基因启动子甲基化
的阳性率为90.9%(86/95)。结论：CCNA1 基因甲基化参与乳腺癌的发生，发展，有淋巴结转移多见的特征,CCNA1 基因甲基化与
乳腺癌转移由关。 |
| 英文摘要: |
| Objective: To investigate the methylation pattern of CCNA1 in breast carcinoma and explore the role of these epigenetic
changes in tumorgenensis. Methods: 95 specimens of breast cancer were used, and the DNA was extracted by Qiagen procedure.
Methylation change in the promoter region of CCNA1 was determined by using restricted enzyme cut-PCR. Results: The positive rate of
gene promoter hypermethylation of CCNA1 was 90.9% (86/95). Conclusions: CCNA1 hypermethylation was correlated with lymph node
metastasis, which may play an important role in pathogenesis of breast cancer. |
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