| 党受琴1 项锋钢1△ 赵永刚2 侯倩1 周旋1.Bmi-1 在非小细胞肺癌中的表达及相关性探讨[J].,2011,11(7):1301-1304 |
| Bmi-1 在非小细胞肺癌中的表达及相关性探讨 |
| Expression of Bmi-1 in NSCLC and Its Relationship to ClinicopathologicCharacters |
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| DOI： |
| 中文关键词: 肺癌 Bmi-1 免疫组织化学 RT-PCR |
| 英文关键词: lung cancer Bmi-1 Immunohistochemistry RT-PCR |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨Bmi-1 在非小细胞肺癌(NSCLC)组织中的表达及其与临床病理特征的关系。方法：采用RT-PCR 检测30 例非小
细胞肺癌及20 例癌旁组织中Bmi-1mRNA 的表达情况，同时应用免疫组织化学SP 法检测52 例非小细胞肺癌及30 例癌旁组织
中Bmi-1 蛋白表达情况。结果：非小细胞肺癌组织中Bmi-1mRNA 表达量明显高于癌旁组织（t=5.188，P<0.01）。肺癌组中Bmi-1 蛋
白的阳性表达率为67.3%（35/52），明显高于癌旁组13.3%（4/30），且表达量差别有高度统计学意义（Z=-4.837,P<0.01）；肺癌组中
Bmi-1 蛋白阳性表达与癌组织的TNM 分期及有无淋巴结转移有关（Z=-2.567,-2.366, P<0.05），而与患者的性别、年龄、组织类型、
分化程度等无关（P 均>0.05）；结论：Bmi-1 在非小细胞肺癌中的高表达与肿瘤发生发展及转移相关，可成为早期诊断肺癌及判断
转移的重要参考指标。 |
| 英文摘要: |
| Objective: To investigate the relationship between the expression of Bmi-1 in nonsmall-cell lung cancer (NSCLC) and
clinicopathologic characters. Methods: Reverse transcription-PCR（RT-PCR）was applied to detect Bmi-1 mRNA in 30 of NSCLC specimen
and 20 of tumor-adjacent tissue. Immunohistochemistry was performed to detect the expression of Bmi-1 in 52 of NSCLC specimen
and 30 of tumor-adjacent tissue. Results: The expression of Bmi-1mRNA in NSCLC specimen was remarkably higher than that in tumor-
adjacent tissue （t=5.188，P<0.01）. The positive rate of Bmi-1 expression was 67.3%（35/52）in NSCLC specimen and 13.3%（4/30）
in tumor-adjacent tissue, respectively. There was a statistically significant difference for the expression of Bmi-1mRNA between NSCLC
specimen and tumor-adjacent tissue （Z=-4.837, P<0.01）. Bmi-1 expression in NSCLC was correlated with both TNM staging and lymph
nodemetastasis（Z=-2.567, -2.366, P<0.05）, butwhich had no relationshipwith sex, age, histotype and differentiation （P >0.05）. Conclusion:
High expression of Bmi-1 in NSCLC is related with the development and metastasis of tumorigenesis. |
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