| 马小娟覃君慧信波王雪莹李颖闫庆国师建国王瑞安.DLC-1 基因在MCF-7 人乳腺癌细胞系中低表达的机制探究[J].,2011,11(7):1231-1234 |
| DLC-1 基因在MCF-7 人乳腺癌细胞系中低表达的机制探究 |
| Study on Mechanism of Low Expression of Tumor Suppressor GeneDLC-1 in Human Breast Cancer Cell Line |
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| DOI： |
| 中文关键词: DLC-1 启动子甲基化 乳腺癌 |
| 英文关键词: DLC-1 Promoter methylation Breast cancer |
| 基金项目:国家自然科学基金（30870923）国家自然科学基金（30971535） |
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| 中文摘要: |
| 目的：探究DLC-1 基因在MCF-7 人乳腺癌细胞系中低表达的机制。方法：应用甲基化特异性PCR(MSP)检测人乳腺癌细胞
MCF-7 的DLC-1 基因甲基化状态，不同浓度的5- 氮杂-2'- 脱氧胞嘧啶(5-Aza-CdR)处理人乳腺癌细胞MCF-7, RT-PCR 及
Real-time PCR 定量检测用药前后细胞中DLC-1 基因mRNA 表达水平变化。结果：DLC-1 基因启动子区CpG 岛呈甲基化状态，经
过5-Aza-CdR 处理后, DLC-1 基因启动子区呈去甲基化状态,并且其mRNA 恢复表达。结论：抑癌基因DLC-1 CpG 岛甲基化是导
致该基因低表达的原因之一, 5-Aza-CdR 能逆转DLC-1 基因甲基化状态。 |
| 英文摘要: |
| Objective: To investigate the mechanism of low expression of tumor suppressor gene dlc-1 in human breast cancer cell
line. Methods:Methylation-specific PCP (MSP) was used to examine the methylation status of DLC-1 promoter.MCF-7 cells were treated
by different concentrations of 5-Aza-CdR. DLC-1 mRNA levels were examined by RT-PCR and Real-time PCR before and after treatment
with 5-Aza-CdR. Results: There was methylation in DLC-1 promoter. The expression of DLC-1 mRNA in MCF-7 increased after treated
by by 5-Aza-CdR. Conclusion:Methylation of DLC-1 promoter might be the main reason of the low expression of DLC-1 inMCF-7 human
breast cancer cells, and the 5-Aza-CdR can inhibit this methylation. |
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