| 张蕾1 刘爱秀2 王强1 李新1 贾济1 王世全1 王百忍1 熊利泽1.氨磷汀对氧糖剥夺引起的PC12 细胞缺血再灌注损伤的保护作用研究[J].,2011,11(6):1110-1113 |
| 氨磷汀对氧糖剥夺引起的PC12 细胞缺血再灌注损伤的保护作用研究 |
| Protection of Amifostine on Oxygen-glucose Deprivation-induced IschemiaReperfusion Injury in PC12 Cells |
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| DOI： |
| 中文关键词: 氨磷汀 脑缺血再灌注损伤 PC12 细胞 氧糖剥夺 凋亡 |
| 英文关键词: Cerebral ischemia reperfusion injury PC12 cell OGD Amifostine Apoptosis |
| 基金项目:国家自然科学基金（30873326）和国家重点基金（30725039）资助项目 |
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| 中文摘要: |
| 目的：研究氨磷汀对体外培养的神经元样细胞的缺血再灌注损伤的保护作用，为其最终用于临床脑缺血的治疗打下基础。
方法：体外培养的PC12 细胞氧糖剥夺4h 后复氧复糖，给予不同浓度的氨磷汀处理，20h 后镜下观察细胞形态学变化，用MTT 和
LDH 检测细胞活力和损伤情况，免疫荧光染色观察凋亡细胞，流式细胞仪计数凋亡细胞的比例。结果：高浓度氨磷汀对正常PC12
细胞活力有抑制作用(P<0.05),而低浓度则无。氨磷汀可以提高缺血再灌注损伤PC12 细胞活力(P<0.05)，减少LDH 释放(P<0.05)，
保护细胞正常形态，抑制细胞凋亡（P<0.05）。结论：氨磷汀对氧糖剥夺引起的神经元样细胞的缺血再灌注损伤具有保护作用。 |
| 英文摘要: |
| Objective: To investigate the effect of amifostine on the ischemia-reperfusion injury in neuron-like PC12 cells. Methods:
PC12 cells were exposed to oxygen-glucose deprivation (OGD) for 4h followed by 20h reoxygenation. Various doses of amfostine were
added into the medium. Cell morphological changes were observed under microscopy and cell viability and integrity were detected by
MTT and LDH techniques. Cell apoptosis was detected by Tunel staining and flow cytometry. Results: High concentration of amfostine
could inhibite PC12 cell viability (P<0.05). Amifostine attenuated the cell viability loss (P<0.05) and LDH leakage (P<0.05) induced by
OGD, protected PC12 cell morphology and decreased cell apoptosis (P<0.05). Conclusion: The amifostine had a protective effect on the
OGD-induced ischemia reperfusion in neuronal cells. |
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