| 袁莉刚刘英黄布敏王睿曲亚玲.一条大鼠睾丸新基因的生物信息学分析及真核表达[J].,2011,11(5):819-823 |
| 一条大鼠睾丸新基因的生物信息学分析及真核表达 |
| Bioinformatic Analysis and Eukaryotic Expression of a NewRat Testicular cDNA Gene |
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| DOI： |
| 中文关键词: 克隆 真核表达 RSA-14-44 基因 生物信息学 |
| 英文关键词: Cloning Prokaryotic expression RSA-14-44 gene Bioinformatics |
| 基金项目:甘肃农业大学创新基金项目（GAU-CX0513） |
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| 中文摘要: |
| 目的：探讨大鼠睾丸组织一条新基因的生物信息学特征和真核表达。方法：构建pEGFP-N1 载体的融合质粒进行真核表达，
利用生物信息学手段分析基因和蛋白功能。结果：生物信息学分析表明RSA14-44 的编码区序列与人类及鼠源RAS 同源基因家
族核酸序列达到85%以上的同源性；RSA14-44 蛋白没有典型的跨膜结构域，也没有典型的N 末端信号肽；与人类RhoA 蛋白序
列达到了89%的同源且具有Rho 家族成员的GAAX 盒和p-loop 结构的基序特征；RSA14-44 蛋白大部分氨基酸序列与Rho 家族
7 个已知结构域高度同源；RSA14-44 基因真核表达定位于细胞质。结论：RSA14-44 基因真核表达定位于CHO-K1 细胞质，；编码
蛋白质与Rho 家族同源性高，为进一步研究其生物学功能提供参考。 |
| 英文摘要: |
| Objective: To investigate the bioinformatic analysis and eukaryotic expression of a new rat testicular cDNA gene.
Methods: The gene was amplified by PCR from genomic DNA of rat testis Genes and was eukaryotic expressed by a recombinant
expression plasmid pEGFP-N1-14,the structure and the function of cloning genes were predicted by bioinformatics. Results: The
homologue of the RSA14-44 gene between the rat and human chromosome RAS family DNA sequence was 85% with and the
homologue of this gene coding protein was 89% with human family RhoA. There were typically GAAX box and p-loop sequence at the
coding region;There were no typical membrane spaning domain and terminal signal peptide. However, the RSA14-44 amino acid
sequence had high homologous with the seven known structural domain of family Rho.The localization for RSA14-44 in CHO cells was
observed in cytoplasm. Conclusion: The coding protein of rat gene was localized in cytoplasm and it was high homologous with Rho
family. |
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