| 王强1 陈宇东1 刘伟英1 王领军1 韩刚1 宋波2.CREB 和NF-κB在p38MAPK所致脊髓星形胶质细胞活化中的作用[J].,2011,11(4):662-667 |
| CREB 和NF-κB在p38MAPK所致脊髓星形胶质细胞活化中的作用 |
| The Role of CREB and NF-κB in the p38MAPK-induced Activationof Spinal Cord Astrocytes |
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| DOI： |
| 中文关键词: 星形胶质细胞 活化 p38MAPK CREB NF-κB |
| 英文关键词: astrocytes activation p38MAPK CREB NF-Κb |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨CREB和NF-κB在p38MAPK所致脊髓星形胶质细胞活化中的作用，明确脊髓星形胶质细胞活化中p38MAPK
细胞信号转导途径的作用。方法：分离培养SPF 大鼠脊髓星形胶质细胞，设正常组、SP 刺激组(SP 组，10-7 mol/L)、SP 刺激
+SB203580(10μmol/L)阻断p38MAPK组(SP+SB组)、SP刺激+ PD98059(10μmol/L)阻断CREB组(SP+PD组)、SP刺激+ SN50
(10μmol/L) 阻断NF-κB (SP+SN组)。WB 法、免疫荧光法、ELISA 法检测12 h 和24 h 时p-p38、p-CREB、NF-κBp65 水平及
GFAP、TNF- 、IL-1β 水平变化。结果：SP组脊髓星形胶质细胞p-p38、p-CREB、NF-κBp65 显著升高，GFAP水平显著增高，同时
TNF-和IL-1β水平显著增高。与SP组比较，用SB203580阻断p38MAPK通路后，SP+SB组p-p38、p-CREB、NF-κBp65 显著降
低，GFAP、TNF-和IL-1β水平显著降低。用PD98059阻断CREB通路后，SP+PD组p-p38、NF-κBp65 无显著变化，p-CREB显著
降低，GFAP 水平降低，同时TNF- 和IL-1β 水平降低。用SN50 阻断NF-κB 通路后，SP+SN 组p-p38、p-CREB 无显著变化，
NF-κBp65 显著降低，GFAP 水平降低，同时TNF- 和IL-1β 水平降低。结论：体外培养中，SP 刺激后脊髓星形胶质细胞显著活
化，p38MAPK活化后通过CREB及NF-κB信号途径导致胶质细胞炎性因子水平显著升高。 |
| 英文摘要: |
| Objective: To investigate the role of CREB and NF-κB in the p38MAPK-induced activation of spinal cord astrocytes,
p38MAPK signal transduction pathway in the activation of spinal cord astrocytes cell. Methods: Aastrocytes, cultured from spinal cord of
SPF rat, were grouped into the normal group, the SP stimulus group (SP group), the SP stimulus + SB203580 interrupt group(SP+SB
group), the SP stimulus + PD98059 interrupt group (SP+PD group) and the SP stimulus + SN50 interrupt group (SP+SN group)in which
SP (10-7mol/L), SB203580 (10 μmol/L), PD98059 (10 μmol/L) and SN50(10 μmol/L) were added to the supernatant for 12h. The
WB method, immunofluorescence method and ELISA method were used to determine the changes of p-p38, p-CREB, NF-κBp65,
GFAP, TNF- , IL-1β of astrocytes or supernatant at 12h and 24h. Results: p-p38, p-CREB, NF-κBp65 level in the SP group increased
significantly. In the same time, GFAP, TNF-a and IL-1β level increased significantly too. When p38MAPK pathway was inhibited by
SB203580 in the SP+SB group, p-p38, p-CREB, NF-κBp65 level and GFAP, TNF-a and IL-1β was significantly reduced compared
with those in the SP group. In the SP+PD group, p-CREB level was significantly reduced compared with those in the SP group. In the
SP+SN group, NF-κBp65 level was significantly reduced compared with those in the SP group. Conclusion: Astrocytes from spinal cord
were significant activated after stimulated by SP in vitro , Inflammatory factors levels from glial cells were significantly increased through
CREB and NF-κB signaling pathways after p38MAPK activation. |
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