| 杜道兵1 卢万根2 曹丛仁3 洪乐鹏1 叶秉坤1 许孟杰1 郝彦利1 冷水龙1.淫羊藿苷促进宫颈癌TC-1细胞凋亡作用的研究[J].,2011,11(4):646-649 |
| 淫羊藿苷促进宫颈癌TC-1细胞凋亡作用的研究 |
| Effect of Icariin in Increasing Apoptosis of Cervical Cancer TC-1 Cells |
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| DOI： |
| 中文关键词: 淫羊藿苷 宫颈癌 TC-1细胞 细胞增殖 细胞凋亡 |
| 英文关键词: Icariin Cervical Cancer TC-1 cell Proliferation Apoptos |
| 基金项目:广东省自然科学基金No.07301608，广州市教委科学基金No.08A036，广州市科技局基金2007J1-C0231资助 |
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| 中文摘要: |
| 目的：研究淫羊藿苷体外对致宫颈癌TC-1细胞的增殖抑制及促凋亡作用。方法：利用细胞培养，用不同浓度的淫羊藿苷在
一定的时间处理致宫颈癌TC-1 细胞，光学显微镜直接观察药物对细胞的作用；MTT 法检测淫羊藿苷对TC-1 细胞的增殖抑制作
用；Dapi核染色、Annexin v-FITC/PI流式细胞学检测细胞凋亡。结果：淫羊藿苷对TC-1细胞有显著的抑制作用，且呈时间、剂量依
赖，20μg/ml作用72小时后，细胞抑制率达99 %；DAPI核染色和流式细胞术检测可发现典型细胞凋亡特征。结论：淫羊藿苷对
TC-1细胞增殖有抑制和促凋亡作用，并呈时间浓度依赖性。 |
| 英文摘要: |
| Objective: To investigate the effect of icariin on proliferation and apoptosis.of TC-1 cells Methods: Based on the culture
of TC-1 cells in vitro, morphological changes of icariin-treated cells were detected by Optical microscope; The effect of icariin on
growth of TC-1 cells was evaluated by MTT assay; DAPI stainin and flow cytometry (Annexin V-FITC/PI) were used to determine the
apoptosis. Results: Optical microscope and MTT assay showed that the icariin had significant antiproliferative effect on TC-1 cells in a
dose-and time-dependent manner significantly. When concentration of the icariin was 20 μg/ml, acting for 72 h, the inhibition rate of
TC-1 cell growth reached to 99%; Features of apoptosis can be found by DAPI nuclear staining and flow cytometry. Conclusions: Icariin
has a significantly effect on inhibiting proliferation and inducing apoptosis of TC-1 cells which was concentration and time dependence. |
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