| 倪兴泰余利鹏△ 韦永中吴昊.福莫特罗对大鼠骨髓间充质干细胞向成骨细胞分化基因表达的影响[J].,2011,11(4):628-631 |
| 福莫特罗对大鼠骨髓间充质干细胞向成骨细胞分化基因表达的影响 |
| Effects of Formoterol on Differentiation of Rat Osteoblast-like Cellsin Vitro |
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| DOI： |
| 中文关键词: β2肾上腺素能受体激动剂 骨髓间充质干细胞 Osterix Runx2 ERK |
| 英文关键词: β2-adrenergic receptor-specific agonist marrow-derived mesenchymal stem cells Osterix Runx2 MEK ERK |
| 基金项目:国家自然科学基金面上项目(30600626) |
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| 中文摘要: |
| 目的：研究β2 肾上腺素能激动剂福莫特罗（Formoterol）对大鼠的体外骨髓间充质干细胞（MMSC）向成骨细胞分化的影响，
进而探讨其作用机制。方法：取SD大鼠的骨髓间充质干细胞，用条件培养液诱导分化后分别加入不同浓度药物，在不同时间点采
用RT-PCR法检测细胞分化中Runx2 和Osterix 的mRNA的表达，采用western blot法检测细胞中MEK和ERK1/2 的磷酸化。结
果：在细胞分化早期，加入已知浓度10-7mol/L 的Formoterol 可抑制成骨样细胞细胞特异性转录因子Runx2 mRNA 表达；在细胞
分化晚期，浓度10-7mol/L的Formoterol也可抑制成骨样细胞Osterix mRNA 表达。在加入浓度10-7mol/ 的Formoterol 作用30min
后，MEK和ERK1/2 的蛋白磷酸化表达均下降。结论：β2 受体激动剂可抑制MMSC细胞体外向成骨样细胞的分化，并且可抑制
MEK和ERK1/2 磷酸化的表达。 |
| 英文摘要: |
| Objective: To observe the effects of Formoterol（β2-adrenergic receptor-specific agonist）on the differentiation of rat
osteoblast-like cells. Methods: Osterix and RUNX2 are osteoblast-specific transcriptional factors that are essential for osteoblast
differentiation and bone formation. RT-PCR was used to detect the Osterix and RUNX2 genes expressions during the treatment of
different drug dose,the expression of P-MEK and P-ERK1/2 were measured by western blot. Results: ①The mRNA expression of cells
cultured with a concentration of 10-7mol/L of Formoterol are obviously low. ②The protein phosphorylation of ERK and MEK in
osteroblasts decreased at 30min treating the cells with Formoterol. Conclusions: In the present study, we examined the molecular
mechanism of Osterix and RUNX2 gene expression, and found that Formoterol inhibited Osterix and RUNX2 expression in the mouse
osteoblast-like cells, Formoterol-induced decrease of Osterix and RUNX2 expression may occurred in dose-dependent manner through
the activation of extracellular signal-regulated kinase pathway. |
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