| 陈博1,2 赵云程1 陈士斌1,2 王静1,2 金贤华1,2 黄俊成1.多因素对昆明小鼠胚胎干细胞原代集落形成和后期增殖的影响[J].,2011,11(4):611-614 |
| 多因素对昆明小鼠胚胎干细胞原代集落形成和后期增殖的影响 |
| Effects of Several Factors on Colony Formation and Post Growthof Generation of Primary Kunming Mouse ESC |
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| DOI： |
| 中文关键词: 胚胎干细胞，细胞因子，温度，氧分压 |
| 英文关键词: ESC Cytokine Temperature Oxygen Partial Pressure |
| 基金项目:国家" 十一五"863计划项目(2008aa101005)，新疆维吾尔自治区高技术研究发展计划项目（200711104）、
新疆畜牧科学院青年科研基金项目（2008QJ01，2010QJ006）资助，中国博士后科学基金项目（200801450） |
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| 中文摘要: |
| 目的：研究细胞因子LIF、EGF、bFGF和肝素钠，以及氧分压、温度等多因素对昆明系小鼠胚胎干细胞（KM-ESC）原代集落形
成和后期增殖的影响。方法：本研究选择LIF、EGF、bFGF、肝素钠、5% O2，20% O2和37℃、39℃作为研究条件。并检测不同情况下
小鼠胚胎干细胞原代集落形成和后期增殖情况。结果：LIF对KM-ESC原代集落形成和后期增殖具有显著促进作用，极显著高于
EGF、bFGF和肝素钠组（P<0.01）。温度对KM-ESC 原代集落形成和增殖具有显著影响，39℃条件下，原代集落形成率、直径和后
期增殖显著高于37℃（P<0.05）；而氧分压对KM-ESC 原代集落形成无显著作用（P>0.05），但是对原代集落直径和后期增殖有一
定促进作用，20% O2组显著高于5% O2组（P<0.05）。结论：LIF、EGF、bFGF、肝素钠、39℃、20% O2对小鼠胚胎干细胞原代集落形
成和后期增殖具有显著促进作用。 |
| 英文摘要: |
| Objective: To investigate the effects of several factors such as cytokines（LIF, EGF, bFGF）, heparin sodium, Oxygen
Stressand, temperature on colony formation and post growth of primary Kunming mouse embryonic stem cell (KM-ESC). Method: LIF,
EGF, bFGF, 5% O2, heparin sodium, 20% O2 Oxygen Stressand and 37℃,39℃ were used in this study. Colony formation and post
growth of primary Kunming mouse embryonic stem cell (KM-ESC) in LIF, EGF, bFGF, heparin sodium, Oxygen Stressand, temperature
were detected. Result: LIF has a significant promoting function on colony formation and post growth of primary KM-ESC, on which the
temperature has a significant effect, higher than on EGF, bFGF and the group of heparin sodium.（P<0.01）Under the condition of 39℃,
the colony formation rate, diameter and post growth are higher than 37℃（P<0.05）. Oxygen Stress has no effect on colony formation of
KM-ESC（P>0.05）, but has a promote effect on colony diameter and post growth. The group of 20% O2 is a little higher than the group of
5% O2（P<0.05）. Conclusion: LIF, EGF, bFGF, 5% O2, heparin sodium, 20% O2 Oxygen Stressand and 39℃ had promoting function on
colony formation and post growth of primary KM-ESC. |
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