陈博1,2 赵云程1 陈士斌1,2 王静1,2 金贤华1,2 黄俊成1.多因素对昆明小鼠胚胎干细胞原代集落形成和后期增殖的影响[J].,2011,11(4):611-614 |
多因素对昆明小鼠胚胎干细胞原代集落形成和后期增殖的影响 |
Effects of Several Factors on Colony Formation and Post Growthof Generation of Primary Kunming Mouse ESC |
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DOI: |
中文关键词: 胚胎干细胞,细胞因子,温度,氧分压 |
英文关键词: ESC Cytokine Temperature Oxygen Partial Pressure |
基金项目:国家" 十一五"863计划项目(2008aa101005),新疆维吾尔自治区高技术研究发展计划项目(200711104)、
新疆畜牧科学院青年科研基金项目(2008QJ01,2010QJ006)资助,中国博士后科学基金项目(200801450) |
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中文摘要: |
目的:研究细胞因子LIF、EGF、bFGF和肝素钠,以及氧分压、温度等多因素对昆明系小鼠胚胎干细胞(KM-ESC)原代集落形
成和后期增殖的影响。方法:本研究选择LIF、EGF、bFGF、肝素钠、5% O2,20% O2和37℃、39℃作为研究条件。并检测不同情况下
小鼠胚胎干细胞原代集落形成和后期增殖情况。结果:LIF对KM-ESC原代集落形成和后期增殖具有显著促进作用,极显著高于
EGF、bFGF和肝素钠组(P<0.01)。温度对KM-ESC 原代集落形成和增殖具有显著影响,39℃条件下,原代集落形成率、直径和后
期增殖显著高于37℃(P<0.05);而氧分压对KM-ESC 原代集落形成无显著作用(P>0.05),但是对原代集落直径和后期增殖有一
定促进作用,20% O2组显著高于5% O2组(P<0.05)。结论:LIF、EGF、bFGF、肝素钠、39℃、20% O2对小鼠胚胎干细胞原代集落形
成和后期增殖具有显著促进作用。 |
英文摘要: |
Objective: To investigate the effects of several factors such as cytokines(LIF, EGF, bFGF), heparin sodium, Oxygen
Stressand, temperature on colony formation and post growth of primary Kunming mouse embryonic stem cell (KM-ESC). Method: LIF,
EGF, bFGF, 5% O2, heparin sodium, 20% O2 Oxygen Stressand and 37℃,39℃ were used in this study. Colony formation and post
growth of primary Kunming mouse embryonic stem cell (KM-ESC) in LIF, EGF, bFGF, heparin sodium, Oxygen Stressand, temperature
were detected. Result: LIF has a significant promoting function on colony formation and post growth of primary KM-ESC, on which the
temperature has a significant effect, higher than on EGF, bFGF and the group of heparin sodium.(P<0.01)Under the condition of 39℃,
the colony formation rate, diameter and post growth are higher than 37℃(P<0.05). Oxygen Stress has no effect on colony formation of
KM-ESC(P>0.05), but has a promote effect on colony diameter and post growth. The group of 20% O2 is a little higher than the group of
5% O2(P<0.05). Conclusion: LIF, EGF, bFGF, 5% O2, heparin sodium, 20% O2 Oxygen Stressand and 39℃ had promoting function on
colony formation and post growth of primary KM-ESC. |
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