| 张春香1△ 罗学刚2 童锦禄3 蒋声海1 符玉梅1 杨道文1.牛肠激酶催化亚基工程菌的构建[J].,2011,11(3):489-492 |
| 牛肠激酶催化亚基工程菌的构建 |
| Building Bovine Enterokinase Catalytic Subunit Engineering Bacteria |
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| DOI： |
| 中文关键词: 牛肠激酶催化亚基 工程菌 构建 |
| 英文关键词: Bovine enterokinase catalytic subunit Engineering bacteria Building |
| 基金项目: |
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| 中文摘要: |
| 目的：构建牛肠激酶催化亚基工程菌。方法：将带有牛肠激酶催化亚基（bEKL）的结构基因进行克隆，将克隆产物纯化后，构
建bEKL克隆质粒，后构建bEKL表达质粒，并将表达质粒制导入备感受态细胞，进行蛋白表达。结果：琼脂糖电泳验证牛肠激酶
催化亚基基因已经成功地插入表达载体，序列与预期设计一致。结论：表达质粒构建成功。 |
| 英文摘要: |
| Objective: To build a Bovine Enterokinase Catalytic Subunit Engineering Bacteria. Methods: Clone the structural gene
of Enterokinase Catalytic Subunit, and construct bEKL clonic plasmid. Then construct bEKL expressive plasmid, and translate the
expressive plasmid into competent cell. Results: The agarose electrophoresis revealed that Bovine Enterokinase Catalytic Subunit gene
had been successfully inserted expressive plasmid and the sequence was same with primary design. Conclusion: The expressive plasmids
was successfully builded. |
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