| 胡超△ 黄丽华左斌谢达平.酵母产γ-氨基丁酸发酵培养基的优化[J].,2011,11(3):482-484 |
| 酵母产γ-氨基丁酸发酵培养基的优化 |
| Optimization of Culture Medium for Gamma-aminobutyricAcid Producedby Saccharomyces Cerevisiae Strain |
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| DOI： |
| 中文关键词: γ-氨基丁酸 酵母 培养基优化 |
| 英文关键词: Gamma-aminobutyric acid Saccharomyces cerevisiae Culture medium optimization |
| 基金项目: |
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| 中文摘要: |
| 目的：提高酵母产γ- 氨基丁酸的能力。方法：采用单因素及正交设计实验对酵母产γ- 氨基丁酸（GABA）的培养基进行优
化。结果：确定最适碳源为葡萄糖，最佳氮源为蛋白胨和硫酸铵复合氮源，合适的无机盐为KH2PO4；最佳发酵培养基为3%葡萄
糖，3%蛋白胨，0.3%(NH4)2SO4和0.1%KH2PO4。在此培养条件下，摇瓶发酵可以获得1.690 g.L-1的GABA产量。结论：发酵培养基
的优化，提高了菌株产γ-氨基丁酸的能力。 |
| 英文摘要: |
| Objective: To improve Gamma-aminobutyric acid (GABA) production by Saccharomyces cerevisiae. Methods: The
culture medium was researched by single factor and orthogonal experiment. Results: The optimum carbon and nitrogen sources were
glucose and the mixture of peptone and (NH4)2SO4, the optimal inorganic salt was KH2PO4. The preferable medium was as follows: 3%
glucose,3% peptone, 0.3% (NH4)2SO4, and 0.1% KH2PO4. Under the optimum conditions, the content of GABA reached 1.690 g.L-1.
Conclusion: The production of GABA was increased under the optimal conditions. |
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