| 孙晓峰1 罗令2 孙启春3 高玉强3 贺又舜1 陈北阳1 吴霞1.左归丸促进小鼠未成熟卵母细胞体外核成熟的实验研究[J].,2011,11(3):444-447 |
| 左归丸促进小鼠未成熟卵母细胞体外核成熟的实验研究 |
| Experimental Study of Zuogui Pills in Stimulation of Mouse ImmatureOocytes Nuclear Maturation in Vitro |
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| DOI： |
| 中文关键词: 左归丸 血清 卵母细胞 生发泡破裂 第一极体 |
| 英文关键词: Zuoguiwan Serum Oocyte Germinal vesicle breakdown Polar body |
| 基金项目:湖南省教育厅资助项目（2009C727）；湖南省科技厅博士后专项（2009RS3018） |
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| 中文摘要: |
| 目的：研究左归丸对小鼠未成熟卵母细胞体外核成熟的影响。方法：制备左归丸含药血清，将生发泡（germinal vesicle，GV）
期卵母细胞分别在不同采血时间获取的左归丸含药血清培养液中进行体外培养，观察左归丸含药血清对生发泡破裂（germinal
vesicle breakdown，GVBD）和第一极体（the first polar body，PB1）排出的时效关系。结果：药物血清组卵母细胞GVBD 的发生率高
于正常血清组和对照组，于培养后4h 差异最显著（P＜0.01）；药物血清组卵母细胞PB1 的发生率高于正常血清组和对照组，于培
养后18h差异最显著（P＜0.01）。结论：2～2.5h左归丸含药血清对未成熟卵母细胞体外核成熟具有明显促进作用。 |
| 英文摘要: |
| Objective: To investigate the effect of Zuoguiwan in vitro on nuclear maturation of immature mouse oocytes.
Methods: The serum containing Zuoguiwan were prepared. The immature oocytes were isolated from the ovaries of the mouse. The GV
oocytes were cultured in the culture media supplemented with Zuoguiwan serum with different blood sampling time, respectively. The
rates of GVBD and PB1 extrusion were detected. Results: There was higer GVBD in several Zuoguiwan serum groups than that in the
controls and normal serum group. And the difference was the most notable at 4th hours. There was higer PB1 in Several Zuoguiwan
serum groups than that in the controls and normal serum group. And the difference was the most notable at 18th hours. Conclusion: 2～
2.5h Zuoguiwan serum improved in vitro nuclear maturation of immature mouse oocytes significantly. |
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