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目的：研究外源性硫化氢（H2S）对创伤失血性休克大鼠炎症反应的影响。方法：选择健康成年雄性SD 大鼠随机分为四组：假手术组（Sham），模型组（HTS），生理盐水组（NS），NaHS 处理组（NaHS），采用创伤失血性休克模型，Sham 组完成所有手术操作，但不放血和复苏，HTS组完成所有手术操作放血后给予Ringer's液复苏，NS组放血后在Ringer's 液复苏前腹腔注射与NaHS 组等容量的生理盐水，NaHS组在复苏前给与NaHS 28μmol/kg（生理盐水稀释至0.5ml）腹腔注射。持续监测各组平均动脉压（MAP）及心律（HR），并通过测定血浆中TNF-α、IL-1β、IL-6 和IL-10 浓度的变化，观察外源性硫化氢对创伤失血性休克大鼠血浆炎症因子的影响。结果：①与HTS组及NS组比较，NaHS组复苏后MAP明显改善（P <0.05）。②与HTS组及NS组比较，复苏后1小时NaHS组血浆TNFα、IL-1β、IL-6浓度明显降低(P < 0.05)；而IL-10浓度四组间差异不明显（P > 0.05）。结论：外源性硫化氢可改善创伤失血性休克大鼠复苏后平均动脉压及抑制复苏后早期炎症反应。

英文摘要:

Objective: To investigate the effects of exogenous hydrogen sulfide (H2S) on inflammatory cytokines in plasma of rats with hemorrhagic traumatic shock. Methods: 40 SD rats weighing 220-250g were randomly divided into four groups (n=10): Sham group, HTS group, NS group, and NaHS group. All animals underwent hemorrhage and resuscitation except Sham group. In NaHS group, 28 μmol/kg NaHS were injected intraperitoneally at the beginning of the resuscitation. In NS group, the rats were administrated the same volume of vehicle at the beginning of the resuscitation. The changes of MAP and HR were recorded at many times. In each group blood samples were collected at 1 hour after resuscitation to determine the concentrations of TNF-α, IL-1β, IL-6 and IL-10 in plasma. Results:①Compared with HTS group and NS group, MAP was improved significantly in NaHS group after resuscitation (P < 0.05) .② At 1 hour after resuscitation, TNF-α, IL-1β and IL-6 in plasma increased significantly in HTS group and NS group compared with Sham group (P <0.05), decreased markedly in NaHS group compared with HTS group and NS group (P < 0.05). The difference of IL-10 among four groups didn't have statistical significance (P > 0.05). Conclusion: Exogenous hydrogen sulfide can improve the MAP after resuscitation, decrease the concentrations of inflammatory cytokines and relieve systemic inflammatory reaction.

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