| 李海建1,2 张广静2 江兰1,2 余春艳1△ 金岩2△.人羊膜间充质细胞分离培养及其干细胞特性研究[J].,2011,11(2):227-229 |
| 人羊膜间充质细胞分离培养及其干细胞特性研究 |
| Study on isolation and culture of human amniotic mesenchymal cellsand its stem cells characteristics |
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| DOI： |
| 中文关键词: 羊膜间充质细胞 细胞培养 干细胞 组织工程 |
| 英文关键词: Human amniotic mesenchymal cells Cell culture Stem cells Tissue engineering |
| 基金项目:国家“863计划”组织工程皮肤重大专项资助项目(2002AA205041，2005AA205241) |
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| 中文摘要: |
| 目的：研究人羊膜间充质细胞( Human amniotic mesenchymal cells,HAMCs)的分离、培养及其干细胞特性，为羊膜间充质细
胞在再生医学的潜在应用奠定实验基础。方法：无菌条件下取正常足月剖腹产胎儿的羊膜剪成碎片，经胰酶胶原酶序贯消化，
DMEM/F12 培养，倒置显微镜下观察其形态，MTT法检测其生长规律，免疫荧光的方法对细胞进行鉴定，定向诱导方法检测细胞
的多向分化潜能。结果：来源于羊膜的间充质细胞，细胞免疫荧光显示SSEA-4,OCT-4阳性，具有很强的增殖能力，并且具有一定
的多向分化能力，在特定条件下可分化为脂肪细胞和成骨细胞；结论：羊膜间充质细胞能够在体外分离、培养、扩增，并且具有干
细胞特性。羊膜间充质细胞在再生医学和组织工程应用有很好的前景。 |
| 英文摘要: |
| Objective: To investigate the isolation, culture and stem cells characteristics of human amniotic mesenchymal cells
(HAMCs)for its potential application in regenerative medicine. Methods:Term placentas from healthy donor mothers obtained from caesarean
sections were minced under sterile conditions. After sequential trypsin and collagenase digestion, cultivated in DMEM/F12, the
morphololgy of HAMCs was observed under the microscope.Growth regularity was observed by MTT assay,the cell was identified by
immunofluorescence, and it has multi-potential ability under specific conditions. Results: Mesenchymal cells derived from human amnion
membrane, SSEA-4 and OCT-4 positive by immunofluorescence, It displays a strong proliferation and multipotential capacity .The cells
can differentiate into fat cells and osteoblasts. Conclusions: HAMCs can isolation , culture and amplification in vitro, and these cells have
stem cell properties . HAMCs has good prospects applications in regenerative medicine and tissue engineering. |
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