| 房定珠1 李晓斌1 廖清奎2 王大斌 马小玉 陈洪波.铁剥夺在TPA 诱导K562 细胞分化中对细胞
生长分化及EGR1mRNA 表达的影[J].,2006,6(5):14-18 |
| 铁剥夺在TPA 诱导K562 细胞分化中对细胞
生长分化及EGR1mRNA 表达的影 |
| K562 Cell Growth and Differentiation and EGR1mRNA Expressionwere Affected by Iron Deprivation During TPA- Induced K562 Cell Differentiation |
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| DOI： |
| 中文关键词: 铁剥夺 细胞分化 EGR1mRNA 细胞周期 细胞表面分化抗原 |
| 英文关键词: Cell differentiation Cell differentiation antigen Cell cycle EGR1mRNA |
| 基金项目:本科题受湖北省教育厅基金资助( NO. 2004D012) |
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| 中文摘要: |
| 目的: 通过TPA 诱导K562 细胞分化过程中干预细胞铁代谢探讨白血病细胞铁与细胞分化的关系及对EGR1mRNA 表
达的影响。方法: 应用体外细胞培养技术通过细胞形态, 细胞化学染色观察细胞生长分化情况; 用FCM、RT- PCR 等技术检细胞
周期、细胞表面分化抗原CD33、CD14 及EGR1mRNA 的表达。结果: 在TPA 诱导K562 细胞分化过程中铁剥夺可明显抑制K562 细
胞生长, 并可阻止TPA 诱导K562 细胞分化, 使K562 细胞停止在S 期。铁剥夺可降低. TPA 诱导K562 细胞分化过程中EGR1mRNA
的表达。讨论: 铁剥夺明显抑制K562 细胞生长、阻止TPA 诱导K562 细胞分化, 故铁剥夺剂( DFO) 可能作为一种辅助抗癌药用于
白血病的化疗, 但由于它能阻止白血病细胞的分化, 故不宜用于白血病的诱导分化治疗。铁剥夺使K562 细胞分化过程中EGR1mRNA
表达降低可能参与了阻止TPA 诱导K562 细胞的分化过程。 |
| 英文摘要: |
| Objective: To investigate the relationship between iron and K562 cells differentiation and EGR1mRNA expression by TPA
inducing K562 cells differentiation and interfering with iron metabolism. Methods: Morphology(Wright. s staining , NSE staining , iron staining)
and flow cytometry were applied to observe the differentiation characteristics of K562 cells, cell- cycle and the differentiation antigen expression of
CD33, CD14 on the surface of cells. PT- PCR was applied to assay the EGR1mRNA expression. Results: The rate of K562 cell growth was 8.
67%, 6. 01% and 98. 88% in k562 + TPA group, TPA+ Fe group and TPA- Fe group respectively. The rate of differentiation- inducing of
K562 was 4. 5% and 95%, 92. 5%, 11% in the control group and other three experiment groups, respectively. K562 cells with NSE staining
showed strong positive in groups of TPA or TPA+ Fe treatment K562 cell, but negative or weak positive in most cells both control group and TPA
- Fe treatment K562 cell group. Inhibiting rate of NaF of K562 was 16. 13%, 58. 72%, 61. 93% and 19. 23% in every group respectively.
Cell cycle remained at S phase in the control group and TPA- Fe group: the percent of S phase cells was 60. 21% and 60. 99% respectively, but
in groups of TPA or TPA+ Fe treatment K562 cell, cell- cycle remained at G0+ G1 phase: the percent of their cells was 51. 7% and 53. 69%
respectively . The expression of CD33 on the surface of cells was similarly 0. 99, 0. 93, 0. 92 and 0. 96 in every group respectively. The expression
of CD33 on the surface of cell was different: in TPA group and TPA+ Fe group ( 0. 31 and 0. 44 respectively) , in control group and TPAFe
group( 0. 05 and 0. 09 respectively) . EGR1mRNA was expressed only in three experiment groups with TPA or TPA+ Fe group or TPA- Fe
group: 1. 0173? 0. 0223, 0. 992? 0. 039 and 0. 4993 ? 0. 028 respectively. Our experiment suggested that iron deprivation inhibited cell growth
and blocked cell differentiation and decreased EGR1mRNA expression, and cell cycle remained at the Speriod during TPA- inducing K562 cell.
Conclusion: K562 cell growth were inhibited and their differentiation were blocked at cell cycle S period, so the agent of iron deprivation could
be regarded as a supplementary anticancer drug in chemical therapy of leukemia. EGR1mRNA expression was reduced by iron deprivation during
TPA- inducing K562 cell differentiation and could participate in blocking K562 cell differentiation course induced by TPA. |
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