| 张燕飞 何 环 师舞阳 万民熙 杨 宇.正交法优化嗜酸氧化亚铁硫杆菌冷冻干燥保护剂[J].,2006,6(5):5-7 |
| 正交法优化嗜酸氧化亚铁硫杆菌冷冻干燥保护剂 |
| Freeze- drying Cryoprotectant Optimized by OrthogonalExperiment for Acidithiobacillus ferrooxidans |
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| DOI： |
| 中文关键词: Q93- 33?? 文献标识码: A |
| 英文关键词: Acidithiobacillus ferroox idans Freeze- dry Cryoprotectant Preserve Orthogonal experiment |
| 基金项目:国家重点基础研究发展计划项目( NO. 2004CB619201) , 国家自然科学基金创新研究群体项目(NO. 50321402) |
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| 中文摘要: |
| 利用正交实验方法, 以甘油、海藻糖、蔗糖和牛血清蛋白为因素, 对嗜酸氧化亚铁硫杆菌( Acidithiobacillus ferrooxidans,
A. ferrooxidans) 冷冻干燥保护剂的最优化配比进行了研究。直观分析、因素指标分析和方差分析的结果表明: 由甘油、海藻糖、蔗
糖和牛血清蛋白组成的冷冻干燥保护剂中, 对存活率影响的主次顺序依次为: 甘油> 海藻糖> 牛血清蛋白> 蔗糖。保护剂的最
优化组合为甘油5%、海藻糖15%、蔗糖18%、牛血清蛋白10%。经过验证, 该组合的保护剂可使冷冻干燥嗜酸氧化亚铁硫杆菌
的存活率达到94%。 |
| 英文摘要: |
| The optimized freeze- drying cryoprotectant of Acidithiobacillus ferrooxidans was studied by orthogonal experiment, in
which glycerol, trehalose, sucrose and bovine serum albumin ( BSA) were regarded as factors. The results of Direct- viewing analysis, the factor
index analysis and variance analysis indicated that in freeze- drying cryoprotectant, which is composed of glycerol, trehalose, sucrose and BSA,
the gradation of influencing survival rate is in turn as follows: glycerol > trehalose > sucrose > BSA. The optimized combination of freezedrying
cryoprotectant consists of 5%( w/ v) glycerol, 15%trehalose, 18%sucrose and 10% BSA. , in which, the survival rate of Acidithiobacil??
lus ferrooxidans could reach 94%. |
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