| 张金伟 曾润颖.南极嗜冷假单胞菌7197 S- 腺苷同型半胱氨酸水解酶
( SAHH) 基因的克隆与序列分析[J].,2006,6(4):5-7 |
| 南极嗜冷假单胞菌7197 S- 腺苷同型半胱氨酸水解酶
( SAHH) 基因的克隆与序列分析 |
| Analysis and Gene Cloning of the S- adenosylhomocysteine Hydrolase fromAntarctic Psychrotrophilic Pseudomonas sp. 7197 Strain |
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| DOI： |
| 中文关键词: 嗜冷假单胞菌 S- 腺苷同型半胱氨酸水解酶 克隆 序列分析 |
| 英文关键词: Psychrotrophilic Pseudomonas S- adenosyl- L- homocysteine hydrolase gene cloning sequence analysis |
| 基金项目:国家自然科学青年基金资助( No. 40406029) |
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| 中文摘要: |
| 从南极普利兹湾深海沉积物中筛选到一株耐冷菌株7197, 其16S rDNA 序列分析表明该菌株属于假单胞菌属( Pseudomonas)
。作者通过设计引物, 从该菌的全基因组DNA 中克隆到编码S- 腺苷- L- 高半胱氨酸( SAHH) 的完整ORF, 全长为
1424bp。使用DNAMAN( 5. 1) 软件对全长ORF 为1424bp 的SAHH 基因进行分析, SAHH 基因编码一个由474AA 残基组成、分子量
预计为52523 Da 的SAHH 蛋白质, 与Psychrobacter sp. 273- 4 的SAHH 有96. 84% 的相似性; 与Acinetobacter sp. ADP1 的SAHH 有
79%的相似性; 与Pseudomonas fluorescens Pf- 5 的SAHH 有75%的相似性。 |
| 英文摘要: |
| A strain 7197, which produces S- adenosyl- L- homocysteine hydrolase, was isolated from the deep sea sediment of
Prydz Bay, Antarctic. The morphology identification and 16S rDNA sequence analysis showed that it belonged to genus Pseudomonas. The Sadenosylhomocysteine
hydrolase genewas cloned by PCR according to the primers. Nucleotide sequence analysis revealed an open reading frame of
1424 bp encoding the SAHH, The amino acid sequence deduced from the nucleotide sequence of the SAHH gene corresponded to a protein of 474
amino acid residues with a molecular weight of 52, 523 kDa. The SAHH shows a high sequence similarity to the SAHH gene from Psychrobacter
sp. 273- 4 and Acinetobacter sp. ADP1, with homolog ies of 96. 84% and 79%, respectively. also show some sequence similarity to the SAHH
gene from Pseudomonas fluorescens Pf- 5 with 75% homologies. |
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