| 青雪梅 李澎涛* * 胡京红 侯金才 华 茜 王 冰 杜 欢.通络救脑注射液对大鼠脑微血管内皮细胞活性
及其条件培养液影响的初步研究[J].,2006,6(4):1-4 |
| 通络救脑注射液对大鼠脑微血管内皮细胞活性
及其条件培养液影响的初步研究 |
| Observing effects of TLJN injection on the rat cerebral microvascularendothelial cells and primary study of the feature of cellular conditioned medium |
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| DOI： |
| 中文关键词: 通络救脑注射液 脑微血管内皮细胞 条件培养液 总蛋白 |
| 英文关键词: TongluoJiunao( TLJN) injection ( TLJN injection) Cerebral microvascular endothelial cells ( CMECs) Conditioned medi??
um Total protein |
| 基金项目:国家重点基础研究发展规划( ?? 973 计划?? ) |
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| 中文摘要: |
| 目的: 观察通络救脑注射液对正常及拟缺血大鼠脑微血管内皮细胞的活性影响, 并初步探讨细胞条件培养液内蛋白
分泌的时效特征、奠定可溶性蛋白深入分析的技术基础。方法: 通络救脑注射液作用于正常及拟缺血脑微血管内皮细胞之后, 用
MTS/ PMS 比色分析法测定细胞的活性, Bradford 法测定细胞培养液总蛋白含量, 比色分析法测定细胞培养液乳酸脱氢酶( LDH) 漏
出值, 同步观察了5 个时间点的细胞活性及条件培养液总蛋白量及LDH 释放量。结果: 通络救脑注射液能够提高拟缺血细胞的
活性, 且抑制LDH 释放量; 正常组分泌总蛋白量3h 达到高峰, 此时细胞活性最佳, LDH 释放量亦少。拟缺血组分泌总蛋白量是
6h 达到高峰, 此时LDH 释放量最少, 但细胞活性与3h 比较有所下降。结论: 通络救脑注射液对拟缺血细胞损伤具有保护作用;
以3h 至6h 的细胞条件培养液做为收集目标是研究条件培养液的最佳时间段。 |
| 英文摘要: |
| Objective: Through observing the active influence of tongluojiunao( TLJN) injection on the normal and ischemic rat cere??
bral microvascular endothelial cells( CMECs) and primary discussion on total protein secretive time- effect feature in cellular conditioned medium,
to establish a technical basis for further study of solubility protein. Methods: After TLJN injection having reacted upon normal and ischemic
cerebral microvascular endothelial cells( CMECs) , MTS/ PMS colorimetric method, Bradford assay and colorimetric analysis antigenic were used
respectively and isochronously to test cellular activity, total protein quantity of conditioned medium, and LDH releasing value of conditioned medi??
um, observing them and analyzing the time- effect feature of cellular conditioned medium. Results: TLJN injection can not only promote the ac??
tivity of ischemic cells, but also reduce LDH releasing value. In the normal group, the peak time of total protein quantity is at 3rdh, at this time,
cell activity is highest and LDH releasing value is least. In the ischemia group, the peak time of total protein quantity is at 6thh, simultaneously,
LDH releasing value is least, but cell activity is a little lower than that of 3rdh. Conclusion: TLJN injection has protective action on ischemic
cells. In this study, it is suggested that regarding conditioned medium from 3rdh to 6thh as collecting aim is the best period to study biological ef??
fects of solubility protein. |
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